Dr. Monica Opiyo
PhD in Biomedical Science
4
Publications
16
Activities
Info
| Name: | Dr. Monica Opiyo |
|---|---|
| Mobile: | +254 709 972 000 |
| E-mail: | mopiyo@daystar.ac.ke |
| Website: | None |
| Updated: | 16 Feb, 2023 |
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About
I have been a teacher at Brookhouse Senior Secondary School and lecturer during my postgraduate training and at Daystar University, School of Science Engineering and Health. In the area of management and administration, I have been co-ordinating Biomedical Science program at Daystar University. Additionally, I possess skills in grant and proposal writing for funding and scholarship, and laboratory and institution collaborative projects. I have at University of Edinburgh, KEMRI Kisian and Wellcome Trust Research Laboratories, Nairobi accrued the following laboratory, biomedical research and analytical skills as outlined below;
In vivo physiology Mouse genotyping, laboratory husbandry of rodents, drug administration via osmotic mini-pump and diet, analysis of in vivo ultrasound of cardiac function, dissection, tail bleeding & plethysmography , surgery and euthanasia of small rodents.
Molecular Biology RNA and DNA extraction, PCR, qRT-PCR including assay design, gel electrophoresis, protein extraction and separation, qRT-PCR arrays, western blotting, experience of reporter gene assays, bacterial transformation, recombinant protein expression and vesicle preparation. Biochemical assays ELISA, Radioimmunoassays, urinalysis, HIV/AIDS using commercial kits. Stool testing using KATO Katz technique, Liquid and gas Chromatography (HPLC-UV)
Cell culture Culture of stable mammalian and bacterial cell lines, culture of human embryonic stem cells ,culture of murine embryonic stem cells and hematopoietic differentiation, experience of plasmid transfection.
Histology Harvesting tissue, fixing, decalcification, cryosectioning, microtoming, immunohistochemistry and tissue staining
Imaging Excellent skills in Light and fluorescence microscopy, experience of confocal and electron microscopy
Software Familiarity with Windows 7, MS Word, MS Excel, MS PowerPoint, MS Outlook, GraphPad Prism, Adobe Photoshop, Image-J analysis and other specialist software packages for image acquisition and experimental analysis (Light Microscopy: MCI Basic 7.0, and Zeiss KS300; Fluorescence microscopy: Q-Capture Pro, Ultrasound: Visualsonics Vevo 770, Western Blotting: LI-COR Odyssey, qRTPCR: Roche Light Cycler 480 SW 1.5,
Field work Extensive experience in data collection ( questionnaire design, focus group discussions, live sample collection) data analysis (EpiInfo, CSpro & SPSS) and evaluation; record keeping; report writing; oral presentations and poster presentations to a variety of audiences.
Academic Qualification
Recent Publications
Teaches
- Human Anatomy
- Medical Physiology
- Histology & Histological Techniques
- Pathology
- Medical Bacteriology
- Medical Virology
- Pharmacology
- Laboratory Animal Husbandry
- Epidemiology & Public Health
- Medical Toxicology
- Medical Mycology
Relevant Links
Publications
Journals
Johnson, JS., Opiyo, MN. Thomson, M, Gharbi, K, Seckl, JR, Heger, A and Chapman, KC.(2016) 11β-hydroxysteroid dehydrogenase-1 alters the gut microbiome response to a high-fat “western” diet." J Endocrinol 232(2):273-283.
Published2016
Link:
Opiyo, MN., Escher, G, Kenyon CJ, Seckl, JR, Andrew, R and Chapman, KC. (2014). "The role of glucocorticoid metabolism in bile acid homeostasis." Endocrine Abstracts 34: 252.
Published2014
Link:
Opiyo, MN, Kenyon, CJ, Manwani, K, Tak, YM, Seckl, JR, Andrew, R and Chapman, KC. (2013). "The role of glucocorticoid metabolism in bile acid homeostasis." Endocrine Abstracts 31: 189
Published2013
Link:
Opiyo, MN., Escher, G, Kenyon, CJ, Andrew, R, Odermatt, A and Chapman, KC. "11b-hydroxysteroid dehydrogenase-1 is required for the synthesis of 7b-hydroxylated bile acids in mice." In preparation.
Published2010
Link:
Activities
Travel Grants
Published2014
Society for Endocrinology Travel Grant to attend BES £500 (2012, 2013 and 2014)
Published2013
University of Edinburgh Global Research Scholarship (2013-2015)
Published2011
Edinburgh Global Overseas Research Scholarship (2011-2012)
Published2011
Charles Darwin Scholarship (2011-2012)
Professional Development Activities
Published2014
2014 Introductory Statistics for Life Scientists (10 module course) Institute for Academic Development, University of Edinburgh
Published2014
2014 Managing bibliographies with Endnote X6 Institute for Academic Development, University of Edinburgh
Published2013
2013 Genetically modified organisms, Disposal of Biological Waste, Compressed gas , Health and Safety Department, University of Edinburgh
Published2012
2012 Introduction to laboratory demonstrating Institute for Academic Development, University of Edinburgh
Published2012
2012 Light microscopy, Workshop Series (16 hours) CALM facility, University of Edinburgh
Published2012
2012 Biosafety, Late and Lone working Health and Safety Department, University of Edinburgh
Published2011
2011 writing a literature review Institute for Academic Development, University of Edinburgh
Published2011
2011 Managing your research project Institute for Academic Development, University of Edinburgh
Published2011
2011 Handling of Radiochemicals, Handling of Liquid Nitrogen Health and Safety Department, University of Edinburgh
Published2011
2011 Writing Up Science, Project Planning and Ethics in Scientific Research, Effective Presentations Transkills Workshops, University of Edinburgh
Published2010
Producing a thesis by word 2010 Institute for Academic Development, University of Edinburgh
Published2010
2010 ScotPIL Training course modules I-IV Home Office Licence training, University of Edinburgh